Description 
Nobilis IB D274 is a live vaccine against Infectious Bronchitis serotype D207 (strain D274) in poultry.

Composition 
Active components per dose:
Live IB strain D274: >=  3.0 log¹⁰ EID₅₀

Formulation 
A freeze-dried pellet.
 
Indications 
Vaccination of future layers and breeding stock. Also as a primer for a booster vaccination with an inactivated IB vaccine containing the same serotype.
 
Vaccination
Vaccination should take place at 8-14 weeks of age. At least two weeks should be allowed to elapse between successive vaccinations with live IB vaccines to avoid interference. If the D274 vaccination is to be followed by an inactivated IB vaccine the optimum booster effect will be obtained if the doses are administered at least 6 weeks apart. In no circumstances should the interval be less than four weeks.

Dosage 
One dose per bird.
 
Method of administration 
The vaccine can be administered by spray, by ocular or nasal instillation or in the drinking water. 

Spray method
The vaccine should be dissolved in cool, clean water which is free of iron and chlorine. The vials should be opened under water. The spray apparatus should be free from sediments, corrosion and traces of disinfectants (preferably used for vaccination purpose only). The vaccine medicated water should be sprayed evenly over the correct number of birds, at a distance of 30 to 40 cm, preferably when the birds are sitting together in dim light. Dissolve 1000 doses in a litre of water and set the nozzle to produce a coarse spray. 

Intranasal / intraocular instillation
Dissolve the vaccine in physiological saline solution (usually 30 ml per 1000 doses) and administer by means of a standardised dropper. One drop should be applied from a height of a few centimetres into one nostril or one eye. The handler should ensure that the nasal drop is inhaled by the bird.
Note: For intranasal / intraocular administration a special diluent, Diluent Oculo Nasal is available. 
  
Drinking water
The vials should be opened under water. Use cool, clean water which is free of iron and chlorine. By adding 2 gm skimmed milk powder per litre of water the virus retains its activity much longer. Ensure uptake of all the vaccine-medicated water in 2 hours. Depending on the weather conditions, it may be advisable to deprive the birds of water prior to vaccination. A sufficient number of water containers to provide adequate drinking space is essential. These should be clean and free from traces of detergents and disinfectants. Dissolve 1000 doses in as many litres of water as the age of the birds in days, to a maximum of 40 litres. The vaccine should be given in the early morning as this is the main period for drinking or the cool period on a hot day. When vaccinating larger flocks, it is advisable to begin by dissolving only part of the vaccine. If vaccine is administered through a central water supply or a proportioner, great care should be taken. For numbers of birds between standard dosages, the next higher dosage should be chosen.

Withholding period 
None

Presentation 
Boxes of vials each containing 1000 or 2500 doses.

Storage 
Store at a temperature of  2-8°C in the dark.

Contra-indications
When used according to instructions: None.
 
Further information
Dispose of empty or part-used vials in accordance with local regulations.

Precautions 

• Wash and disinfect hands and equipment after vaccinating. 
• Vaccinate only healthy birds. 
• Each vial should be used immediately after opening.